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Color Taq PCR Master Mix (2x)

Color Taq PCR Master Mix (2x) is a ready-to-use solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 , dNTPs and two gel tracking dyes.

Use of Color Taq PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR set-up.

Taq DNA Polymeraseis a thermostable enzyme of approximately 94 kDa from Thermus aquaticus.

Ultrapure, recombinant protein.

The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C (1,2).

Catalyzes the polymerization of nucleotides into duplex DNA in the 5′->3′ direction in the presence of magnesium ions.

Maintains the 5′->3′ exonuclease activity.

Lacks the 3′->5′ exonuclease activity.

Adds extra A at the 3′ ends.

Color Taq PCR Master Mix (2x) is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.

Color Taq PCR Master Mix (2x) contains:

  1. Color Taq PCR Master Mix (2x)
  2. Water, nuclease free