Color Taq PCR Master Mix (2x) is a ready-to-use solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 , dNTPs and two gel tracking dyes.
Use of Color Taq PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR set-up.
Taq DNA Polymeraseis a thermostable enzyme of approximately 94 kDa from Thermus aquaticus.
Ultrapure, recombinant protein.
The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C (1,2).
Catalyzes the polymerization of nucleotides into duplex DNA in the 5′->3′ direction in the presence of magnesium ions.
Maintains the 5′->3′ exonuclease activity.
Lacks the 3′->5′ exonuclease activity.
Adds extra A at the 3′ ends.
Color Taq PCR Master Mix (2x) is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.
Color Taq PCR Master Mix (2x) contains: