Taq PCR Master Mix (2x) is a ready-to-use solution containing Taq DNA Polymerase, optimized reaction buffer, MgCl2 and dNTPs.
Use of Taq PCR Master Mix (2x) allows to save time and reduce contamination risk due to fewer pipetting steps during PCR set-up.
Taq DNA Polymeraseis a thermostable enzyme of approximately 94 kDa from Thermus aquaticus.
Ultrapure, recombinant protein.
The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C (1,2).
Catalyzes the polymerization of nucleotides into duplex DNA in the 5´->3´ direction in the presence of magnesium ions.
Maintains the 5´->3´ exonuclease activity.
Lacks the 3´->5´ exonuclease activity.
Adds extra A at the 3′ ends.
Taq PCR Master Mix (2x) is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.
Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 min at 74°C. The reaction conditions are: 50 mM Tris-HCl (pH 9.0 at 25°C), 50 mM NaCl, 5 mM MgCl2, 200 μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10 μg activated calf thymus DNA and 0.1 mg/ml BSA in a final volume of 50 μl.
Storage Conditions: Store at -20°C for long-term storage or at 4°C for up to 2 months.
Taq PCR Master Mix (2x) contains:
Taq PCR Master Mix (2x): Taq DNA Polymerase is supplied in 2 x Pol Buffer B containing 3 mM MgCl2 and 0.4 mM of each dNTP.
۱۰ x Color Load: contains two gel tracking dyes and a gel loading reagent. It enables direct loading of PCR products onto an agarose gel.
Quality Control: All preparations are assayed for contaminating endonuclease, 3′-exonuclease, and nonspecific single- and double-stranded DNase activities. Typical preparations are greater than 95% pure, as judged by SDS polyacrylamide gel electrophoresis