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MP qPCR Master Mix (2x)

MP qPCR Master Mix (2x) is a universal solution for quantitative, multiplex real-time PCR and two-step real-time RT-PCR using sequence-specific probes and can be used on most real-time PCR cyclers available. Depending on the real-time cycler used, up to 5 targets can be quantified simultaneously in the same well or tube. The master mix contains Perpetual Taq DNA Polymerase, optimized reaction buffer, dNTPs (dTTP is partially replaced with dUTP). The polymerase enables convenient room temperature reaction setup. MP qPCR Master Mix (2x) contains dUTP, which partially replaces dTTP. It allows the optional use of a uracil-N-glycosylase (UNG) to prevent carryover contamination between reactions. UNG removes uracil from any dU-containing contaminating amplicons, leaving abasic sites and making DNA molecules susceptible to hydrolysis during the initial denaturation step