Probe qPCR Master Mix (2x) is a universal solution for quantitative real-time PCR and two-step real-time RT-PCR and can be used on most real-time PCR cyclers available.
The master mix contains Perpetual Taq DNA Polymerase, optimized reaction buffer, dNTPs (dTTP is partially replaced with dUTP).
Perpetual Taq DNA Polymerase contains recombinant Taq DNA Polymerase bound to anti-Taq monoclonal antibodies that block polymerase activity at moderate temperatures.
The polymerase activity is restored during the initial denaturation step when amplification reactions are heated at 95°C for at least two minutes.
Use of the “hot start” enzyme prevents extension of misprimed products and primer-dimers during reaction setup leading to higher specificity and sensitivity of PCR reactions.
The polymerase enables convenient room temperature reaction setup.
Probe qPCR Master Mix (2x) contains dUTP, which partially replaces dTTP. It allows the optional use of a uracil-N-glycosylase (UNG) to prevent carryover contamination between reactions. UNG removes uracil from any dU-containing contaminating amplicons, leaving abasic sites and making DNA molecules susceptible to hydrolysis during the initial denaturation step.