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Color Taq DNA Polymerase

Stable thermophilic DNA polymerase, suitable for applications requiring high temperature synthesis of DNA.

The enzyme is supplemented with two inert gel tracking dyes.

Description:

  • Taq DNA Polymeraseis a thermostable enzyme of approximately 94 kDa from Thermus aquaticus.
  • Ultrapure, recombinant protein.
  • The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C (1,2).
  • Catalyzes the polymerization of nucleotides into duplex DNA in the 5´->3´ direction in the presence of magnesium ions.
  • Maintains the 5´->3´ exonuclease activity.
  • Lacks the 3´->5´ exonuclease activity.
  • Adds extra A at the 3′ ends.
  • Color Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.
  • Use of Color Taq DNA Polymerase offers several advantages:
  • visualizes the addition of the polymerase to the reaction,
  • confirms complete mixing,
  • enables direct loading of PCR products onto an agarose gel without addition of a gel loading buffer,
  • the added dyes allow to track electrophoresis progress,
  • do not affect PCR performance,
  • do not interfere with most downstream applications (exeption: the polymerase is not recommended for any downstream application using absorbance or fluorescence excitation).