Native, very stable thermophilic DNA polymerase, suitable for applications requiring high temperature synthesis of DNA.
Source: Thermus aquaticus
Taq DNA Polymeraseis a thermostable enzyme of approximately 94 kDa from Thermus aquaticus.
The enzyme replicates DNA at 74°C and exhibits a half-life of 40 min at 95°C.
Catalyzes the polymerization of nucleotides into duplex DNA in the 5´->3´ direction in the presence of magnesium ions.
Maintains the 5´->3´ exonuclease activity.
Lacks the 3´->5´ exonuclease activity.
Taq DNA Polymerase is recommended for use in PCR and primer extension reactions at elevated temperatures to obtain a wide range of DNA products up to 10 kb.
Native Taq DNA Polymerase is recommended for use in special PCR applications, where traces of E.coli genomic DNA may interfere with amplification specificity.
Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 min at 74°C. The reaction conditions are: 50 mM Tris-HCl (pH 9.0 at 25°C), 50 mM NaCl, 5 mM MgCl2, 200 μM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [³H]dTTP), 10 μg activated calf thymus DNA and 0.1 mg/ml BSA in a final volume of 50 μl.